Knowledge and attitudes toward anaphylaxis to local anesthetics in dental practice.

OBJECTIVE/AIM: The absence of a comprehensive understanding of potential anaphylactic reactions to local anesthetics (LAs) and management can result in grave consequences. For this reason we aim to assess Latin American dentists' knowledge, preparedness, and competency in managing anaphylactic reactions to LAs. MATERIALS AND METHODS: Design: A cross-sectional study was conducted from November 2021 to February 2022. Board-certified dentists answered a survey comprising 26 structured questions. Chi-square tests and logistic regression models were performed in Stata 17.0. SETTING: Argentina, Brazil, Colombia, Costa Rica, Ecuador, Honduras, Mexico, Peru, Venezuela, and other Latin American countries. RESULTS: Of 507 respondents, lidocaine was the most frequently used LA (88.1%). While 85.2% could identify dyspnea as a symptom of anaphylaxis, only 50.1% knew the correct route for epinephrine administration, and just 43.5% had epinephrine in their emergency kits. Confidence in managing anaphylactic reactions was low (9.6%). Older age was inversely related to both knowledge of anaphylaxis management and the possession of epinephrine (P = 0.003 and P = 0.0001, respectively). DISCUSSION: Our study highlights a concerning discrepancy between the practical readiness of Latin American dentists in handling anaphylaxis. CONCLUSION: The study's findings underscore the need for educational interventions to improve the readiness to identify and handle anaphylactic emergencies in dental practice.

O cuidado ao paciente com câncer sob a ótica de enfermeiros da atenção primária à saúde / Cancer patient care from the perspective of primary health care nurses / La atención al paciente oncológico desde la perspectiva de los enfermeros de atención primaria a la salud

RESUMO Objetivo: descrever a percepção de enfermeiros sobre o cuidado ao paciente com câncer na Atenção Primária à Saúde. Método: estudo qualitativo, desenvolvido em 26 Centros de Saúde da Família de um município do Oeste catarinense - Brasil, entre setembro e outubro de 2022. Participaram 33 enfermeiros, que responderam um questionário on-line. O texto foi interpretado com base nos preceitos conceituais da cultura organizacional da teoria administrativa de Chiavenatto. Questões objetivas foram analisadas pela frequência das respostas; para as dissertativas, utilizou-se o Discurso do Sujeito Coletivo. Resultados: os enfermeiros percebem-se despreparados e identificam ausência de protocolos e fluxos organizacionais. Considerações finais: na Atenção Primária à Saúde há a crença consolidada no cotidiano laboral e que se torna integrante da cultura organizacional dos serviços de saúde, que o paciente com câncer deve seguir para o cuidado especializado. Nota-se desconhecimento das políticas de saúde, o que implica o profissional na atenção ao paciente com câncer.

ABSTRACT Objective: To describe nurses' perceptions of cancer patient care in Primary Health Care. Method: A qualitative study was conducted in 26 Family Health Centers in a municipality in western Santa Catarina - Brazil, between September and October 2022. A total of 33 nurses took part and completed an online questionnaire. The text was interpreted based on the conceptual precepts of organizational culture from Chiavenatto's administrative theory. The frequency of responses analyzed objective questions; the Collective Subject Discourse was used for the essay questions. Results: Nurses perceive themselves as unprepared and identify a lack of protocols and organizational flows. Final considerations: In primary Health Care, there is a belief consolidated in everyday work and becomes part of the organizational culture of health services, that cancer patients should go on to specialist care. There is a lack of knowledge of health policies, which implicates professionals in caring for cancer patients.

RESUMEN Objetivo: describir las percepciones de los enfermeros sobre la atención al paciente oncológico en Atención Primaria. Método: estudio cualitativo realizado en 26 Centros de Salud de la Familia de un municipio del oeste de Santa Catarina - Brasil, entre septiembre y octubre de 2022. Participaron 33 enfermeros que cumplimentaron un cuestionario en línea. El texto se interpretó basándose en los preceptos conceptuales de la cultura organizativa de la teoría administrativa de Chiavenatto. Las preguntas objetivas se analizaron por la frecuencia de las respuestas; para las preguntas de ensayo, se utilizó el discurso del sujeto colectivo. Resultados: los enfermeros no se sienten preparados e identifican una falta de protocolos y flujos organizativos. Consideraciones finales: en Atención Primaria existe la creencia, consolidada en el trabajo diario y que se ha convertido en parte integrante de la cultura organizativa de los servicios sanitarios, de que los pacientes con cáncer deben pasar a la atención especializada. Existe un desconocimiento de las políticas sanitarias, que implica a los profesionales en la atención a los pacientes con cáncer.

Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil.

The main objective of this study was to investigate the dynamic of SARS-CoV-2 viral excretion in rectal swab (RS), saliva, and nasopharyngeal swab (NS) samples from symptomatic patients and asymptomatic contacts. In addition, in order to evaluate the replication potential of SARS-CoV-2 in the gastrointestinal (GI) tract and the excretion of infectious SARS-CoV-2 from feces, we investigated the presence of subgenomic nucleoprotein gene (N) mRNA (sgN) in RS samples and cytopathic effects in Vero cell culture. A prospective cohort study was performed to collect samples from symptomatic patients and contacts in Rio de Janeiro, Brazil, from May to October 2020. One hundred and seventy-six patients had samples collected at home visits and/or during the follow up, resulting in a total of 1633 RS, saliva, or NS samples. SARS-CoV-2 RNA was detected in 130 (73.9%) patients who had at least one sample that tested positive for SARS-CoV-2. The presence of replicating SARS-CoV-2 in RS samples, measured by the detection of sgN mRNA, was successfully achieved in 19.4% (6/31) of samples, whilst infectious SARS-CoV-2, measured by the generation of cytopathic effects in cell culture, was identified in only one RS sample. Although rare, our results demonstrated the replication capacity of SARS-CoV-2 in the GI tract, and infectious viruses in one RS sample. There is still a gap in the knowledge regarding SARS-CoV-2 fecal-oral transmission. Additional studies are warranted to investigate fecal or wastewater exposure as a risk factor for transmission in human populations.

Genetic and biochemical characterization of BIM-1, a novel acquired subgroup B1 MBL found in a Pseudomonas sp. strain from the Brazilian Amazon region.

OBJECTIVES: To characterize a novel acquired MBL, BIM-1, in a Pseudomonas #2 (subgroup P. guariconensis) strain isolated from the Aurá river located in the Brazilian Amazon hydrographic basin. METHODS: WGS using an Illumina® MiSeq System was used to characterize the genome of Pseudomonas sp. IEC33019 strain. Southern blotting/hybridization assays were performed to confirm the location of the MBL-encoding gene, blaBIM-1 (Belém Imipenemase). Antimicrobial susceptibility testing, cloning, and biochemical and phenotypic characterization were performed to determine BIM-1 kinetics. RESULTS: The IEC33019 strain showed high resistance rates to ß-lactams, ciprofloxacin and aminoglycosides, being susceptible only to polymyxins and susceptible, increased exposure to aztreonam. WGS analysis revealed a novel acquired MBL-encoding gene, blaBIM-1, found as a gene cassette inserted into a class 1 integron (In1326) that also carried qnrVC1 and aadA11e. In1326 was located in a complex transposon, Tn7122, carried by a 52.7 kb conjugative plasmid (pIEC33019) with a toxin/antitoxin system (vapB/vapC). BIM-1 belongs to the molecular subgroup B1 and shares 70.2% and 64.9% similarity with SIM-1 and IMP-1, respectively. Kinetics analysis of BIM-1 showed hydrolytic activity against all ß-lactams tested. CONCLUSIONS: BIM-1 is a novel acquired MBL encoded by a gene carried by mobile genetic elements, which can be transferred to other Gram-negative bacilli (GNB). Because the IEC33019 strain was recovered from a river impacted by a populous metropolitan region with poor basic sanitation and served by limited potable freshwater, it would be important to establish the role of the BIM-1-producing GNB as nosocomial pathogens and/or as colonizers of the riverside population in this geographical region.

Qualidade da dieta e fatores associados em pacientes adultos e idosos atendidos em um ambulatório de nutrição na cidade de Recife, Pernambuco / Diet quality and associated factors in adult and elderly patients treated in a nutrition outpatient clinic in the city of Recife, Pernambuco

O consumo alimentar atual, caracterizado pelo aumento da ingestão de alimentos de alta densidade calórica e baixo valor nutricional pode influenciar negativamente no estado nutricional e surgimento de agravos à saúde. Este estudo teve como objetivo avaliar a qualidade da dieta e fatores associados em pacientes adultos e idosos atendidos num ambulatório de nutrição. Trata-se de um estudo transversal realizado de maio a outubro de 2021 no ambulatório de nutrição do Instituto de Medicina Integral Prof. Fernando Figueira em Recife, Pernambuco. Foram coletadas as informações do perfil sociodemográfico e econômico, estilo de vida, variáveis clínicas e estado nutricional, no momento do atendimento nutricional (primeira consulta e/ou consulta de acompanhamento). A avaliação do consumo alimentar foi realizada por meio da análise do Índice de Qualidade da Dieta Revisado (IQDR). A amostra foi composta por 102 pacientes, e foi evidenciada uma maior frequência de excesso de peso/obesidade e risco cardiovascular muito elevado. Com relação ao IQDR, 72,6% da amostra apresentam dieta saudável. O tempo de acompanhamento foi correlacionado positivamente com a qualidade da dieta (ρ 0,199; p<0,045); e quanto à glicemia de jejum, houve uma correlação negativa com o consumo de cereais totais (ρ -0,229; p<0,050), e positiva com o consumo de leite e derivados (ρ0,265; p<0,023). No que se refere às variáveis antropométricas, evidenciou-se correlações negativas com o consumo de hortifrútis e cereais integrais. O consumo alimentar é considerado complexo e necessita da avaliação dos fatores que possam interferir no mesmo. O acompanhamento nutricional, a modificação e a melhora da qualidade da alimentação, favorecem à adoção de um estilo de vida mais saudável, relacionando-se com a prevenção e/ou o controle das doenças crônicas não transmissíveis e suas complicações.

Current food consumption, characterized by increased intake of high-density foods and low nutritional value can negatively influence nutritional status and the emergence of health problems. This study aimed to evaluate the quality of the diet and associated factors in adult and elderly patients treated in a nutrition outpatient clinic. This is a cross-sectional study conducted from May to October 2021 at the Nutrition Outpatient Unit of the Fernando Figueira Institute of Integral Medicine in Recife, Pernambuco. The information of the sociodemographic and economic profile, lifestyle, clinical variables, and nutritional status was collected at the time of nutritional consultation (first consultation and/or follow-up consultation). The evaluation of food consumption was performed through the analysis of the Diet Quality Index - Revised (DQI-R). The sample consisted of 102 patients, and there was a higher frequency of overweight/obesity and very high cardiovascular risk. Regarding DQI-R, 72.6% of the sample has a healthy diet. The follow-up time was positively correlated with the quality of the diet (ρ 0.199; p <0.045); and regarding fasting blood glucose, there was a negative correlation with the consumption of total cereals (ρ -0.229; p <0.050) and a positive correlation with the consumption of milk and derivatives (ρ 0.265; p <0.023). Regarding anthropometric variables, negative correlations with the consumption of whole grains and cereals was demonstrated. Food consumption is considered complex and requires the evaluation of factors that may interfere with it. Nutritional monitoring, modifying, and improving the quality of food, favors the adoption of a healthier lifestyle, which relates to the prevention and/or control of chronic non-communicable diseases and their complications.

Factores asociados a la satisfacción estudiantil con docentes, infraestructura e investigación en escuelas médicas latinoamericanas

Introducción: La satisfacción estudiantil con los programas académicos es importante en la educación médica, para crear futuros galenos competentes. No hay estudios latinoamericanos multicéntricos que determinen sus factores asociados, pare mejorar el currículo médico. Objetivo: Determinar el porcentaje y factores asociados a la satisfacción estudiantil con docentes, infraestructura e investigación en 9 escuelas médicas latinoamericanas. Métodos: Estudio transversal descriptivo de datos secundarios obtenidos mediante cuestionario administrado a estudiantes de medicina de 9 universidades latinoamericanas; indagó variables socioeducativas y la satisfacción estudiantil respecto a docentes, infraestructura e investigación de las universidades. Para el análisis se usaron modelos lineales generalizados. Resultados: De 2 649 encuestados, 57 % eran mujeres con mediana de 22 años. Menos del 50 % estuvo satisfecho con infraestructura e investigación; solo frente a docencia hubo 66 % de satisfacción; Colombia fue el de mayor satisfacción con los docentes, con 82 % de encuestados satisfechos, mientras que Chile fue el país más satisfecho respecto a la infraestructura e investigación universitaria, con 67 % y 68 %, respectivamente. Estudiar en universidad privada se asoció a satisfacción con docentes (p= 0,002), infraestructura (p= 0,001) e investigación (p= 0,018), esta última mayor en mujeres (p= 0,033), pero menor en quienes sentían que les alcanzaba el dinero del mes (p= 0,001). Conclusiones: La satisfacción médico-estudiantil en universidades latinoamericanas tiende hacia la baja, están más satisfechos los de universidades privadas, tienen mayor satisfacción frente a la investigación las mujeres y menor quienes sienten que les alcanza el dinero hasta fin de mes.

Introduction: Student satisfaction with academic programs is important in medical education to create future competent doctors. There are not multicenter Latin American studies that determine its factors related, to improve medical curricula. Objective: To determine the percentage and factors associated with student satisfaction with teachers, infrastructure, and research in 9 Latin American medical schools. Methods: Descriptive cross-sectional study of secondary data obtained through an administered questionnaire to medical students from 9 Latin American universities between 2016-2017, which inquired about socio-educational variables and student satisfaction regarding teachers, infrastructure, and university research. Generalized Linear Models-GLM was used for the analysis. Results: Of 2 649 surveyed, 57% were women, the median was 22 years. Less than 50% were satisfied with infrastructure and research, only compared to teaching there was 66% satisfaction, Colombia ranking as the one with the highest satisfaction with teachers, with 82% of satisfied respondents, while Chile was the most satisfied country regarding infrastructure and university research with 67% and 68%, respectively. Studying at a private university was associated with teacher satisfaction (p= 0.002), infrastructure (p= 0.001) and research (p= 0.018), the latter was higher in women (p= 0.033), but lower in those who felt that the money of the month was reaching them (p= 0.001). Conclusions: Medical-student satisfaction with Latin American universities is tending downward, with those studying at private universities being more satisfied, women having greater satisfaction with research and less for those who feel that they have enough money until the end of the month.

Desfecho clínico e fatores associados em pacientes comCOVID-19 internados em uma Unidade de Terapia Intensiva / Clinical outcome and associated factors in patients with COVID-19 admittedto an Intensive Care Unit

Introdução: O Corona Virus Disease (COVID-19) é umainfecção de elevada transmissibilidade e está associada commaior suscetibilidade ao desenvolvimento de desnutrição.Desta forma, a terapia nutricional enteral deve estar inte-grada ao tratamento global deste agravo. Objetivo: Analisar os fatores clínicos, nutricionais e bioquí-micos associados ao desfecho clínico de pacientes com COVID-19 internados em uma Unidade de Terapia Intensiva (UTI). Métodos: Trata-se de uma série de casos, realizado no pe-ríodo de março a julho de 2020, com os dados registrados nasfichas de acompanhamento nutricional dos pacientes interna-dos com COVID-19. As variáveis analisadas foram: demográ-ficas (idade e sexo); clínicas (comorbidades, doença de basee desfecho), antropométricas, bioquímicas e de suporte nu-tricional. Resultados: A amostra foi composta por 102 pacientes,65,7% apresentaram síndrome respiratória aguda grave, aprincipal doença de base observada foi as doenças cardíacas(23,5%) e 69,3% dos pacientes evoluíram para a alta da UTI.Com relação às variáveis nutricionais, aproximadamente 50%dos pacientes receberam alimentação por via enteral e 73,3%iniciaram de forma precoce. No que se refere aos marcadores bioquímicos, os pacientes que foram a óbito, apresentarammaiores valores das razões proteína C reativa (PCR) /albu-mina (p=0,024) e das concentrações de PCR (p=0,012)quando comparado com os que evoluíram para alta da UTI.Além disso, observa-se que os idosos (HR ajustada =3,62;IC95% 1,19 – 10,97) e o início precoce da terapia nutricionalenteral (HR ajustada = 10,62; IC95% 2,41 – 46,87) foram fa-tores relacionados com a alta da UTI. Conclusão: O acompanhamento do processo inflamatórioatravés de diferentes marcadores parece ser um bom parâ-metro de evolução clínica desses pacientes. Além disso, osbenefícios da terapia de nutrição enteral precoce podem es-tar associados à melhor evolução clínica e redução de com-plicações durante internamento.(AU)

ntroduction: The Corona Virus Disease (COVID-19) is ahighly transmissible infection and is associated with greatersusceptibility to the development of malnutrition. Thus, en-teral nutritional therapy must be integrated into the globaltreatment of this condition. Objective: To analyze the clinical, nutritional and biochem-ical factors associated with the clinical outcome of patientswith COVID-19 admitted to an Intensive Care Unit (ICU). Methods: This is a case series, carried out from March toJuly 2020, with data recorded in the nutritional monitoringrecords of patients hospitalized with COVID-19. The variablesanalyzed were: demographic (age and sex); clinical (comor-bidities, underlying disease and outcome), anthropometric,biochemical and nutritional support. Results: The sample consisted of 102 patients, 65.7% hadthe severe acute respiratory syndrome, the main underlyingdisease observed was heart disease (23.5%) and 69.3% ofpatients were discharged from the ICU. Regarding nutritionalvariables, approximately 50% of patients received enteralfeeding and 73.3% started early. Concerning biochemicalmarkers, patients who died had higher C-reactive protein(CRP)/albumin ratios (p=0.024) and CRP concentrations(p=0.012) when compared to those who progressed to dis-charge from the ICU. In addition, it is observed that the eld-erly (adjusted HR = 3.62; 95%CI 1.19 – 10.97) and early ini-tiation of enteral nutritional therapy (adjusted HR = 10.62;95%CI 2.41 – 46 .87) were factors related to ICU discharge. Conclusion: Monitoring the inflammatory process usingdifferent markers seems to be a good parameter for theclinical evolution of these patients. In addition, the benefitsof early enteral nutrition therapy may be associated withbetter clinical outcomes and reduced complications duringhospitalization.(AU)

Índice CPOD y ceo-d en niños de 5 a 8 años de una escuela en una localidad de Ecuador / CPOD and ceo-d index in children between 5 and 8 years old from a school in a locality in Ecuador

Las enfermedades orales son las más comunes de las enfermedades crónicas y uno de los problemas más importantes de salud pública debido a su prevalencia, su impacto en los individuos y la sociedad y el costo de su tratamiento. En el caso de los niños este efecto se multiplica por las consecuencias que se derivan para la edad adulta. El objetivo de este estudio fue estimar los índices ceo-d en niños escolares de 5 a 8 años en la Unidad Educativa "Alejandro Dávalos Calle", Ecuador. Para ello, bajo una investigación exploratoria, descriptiva, no experimental, de campo y de corte transversal. Se seleccionó como población los niños entre 5 y 8 años de edad en el periodo académico 2017-2018; la muestra estuvo conformada por 96 niños de ambos sexos, previo a consentimiento informado y cumpliendo las normas de bioéticas. Se realizaron revisión de historia clínica de los infantes y se les aplico encuestas validadas por expertos, a estudiantes, representantes y docentes. Se obtuvo prevalencia a caries en el 100% de los niños, 47% con dientes obturados y 64% perdieron piezas dentales, no se encontró evidencia estadísticamente significativa entre la presencia de caries y el sexo. El CEOD moderado, identificándose como factores de riesgos el desconocimiento de las medidas higiénicas bucal, baja frecuencia en la revisión odontológica y una dieta altamente cariogénica en estos niños. Se recomienda diseñar campañas educativas integrales que oriente a buenas prácticas de higiene bucal con el objetivo de prevenir y minimizar el número de personas afectadas por este mal(AU)

Oral diseases are the most common of the chronic diseases and one of the most important public health problems due to their prevalence, their impact on individuals and society, and the cost of their treatment. In the case of children, this effect is multiplied by the consequences that are derived for adulthood. The objective of this study was to estimate the ceo-d indices in school children aged 5 to 8 years at the "Alejandro Dávalos Calle" Educational Unit, Ecuador. For this, under an exploratory, descriptive, non-experimental, field and cross-sectional investigation. Children between 5 and 8 years of age in the academic period 2017-2018 were selected as the population; The sample consisted of 96 children of both sexes, prior to informed consent and complying with bioethical norms. The infants' clinical history was reviewed and surveys validated by experts, students, representatives, and teachers were applied. Caries prevalence was obtained in 100% of the children, 47% with filled teeth and 64% lost teeth, no statistically significant evidence was found between the presence of caries and sex. The CEOD is high, identifying as risk factors the ignorance of oral hygiene measures, low frequency in dental check-ups and a highly cariogenic diet in these children. It is recommended to design comprehensive educational campaigns that guide good oral hygiene practices in order to prevent and minimize the number of people affected by this disease(AU)

SARS-CoV-2 variant N.9 identified in Rio de Janeiro, Brazil.

BACKGROUND: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) B.1.1.33-derived lineage named N.9 was described recently in Brazil and it's considered a potential variant of interest (VOI) due to the presence of E484K substitution at the receptor-binding domain (RBD) of the Spike (S) protein. OBJECTIVE: To describe the first detection of variant N.9 in Rio de Janeiro State. METHODS: SARS-CoV-2 N.9 was confirmed by quantitative reverse transcription polymerase chain reaction (qRT-PCR), whole-genome sequencing and phylogenetic analysis. FINDINGS: Here, we report two SARS-CoV-2 N.9 lineage strains in Rio de Janeiro. One of them had only the E484K substitution of the six N.9 lineage-defining mutations. Other three strains pre-defined as N.9 have the same genomic profile. These four strains are grouped within the B.1.1.33 lineage and basal to the N.9 lineage in our phylogenetic analysis, and we call them "N.9-like/B.1.1.33 + E484K". MAIN CONCLUSIONS: The phylogenetic analysis shows four independent introductions of N.9 in the state of Rio de Janeiro in October and December 2020, January and March 2021. SARS-CoV-2 N.9 dissemination in the Rio de Janeiro could have been limited by the emergence and dominance of other variants, mainly by the lineage P.2 VOI Zeta that emerged in the same period and co-circulated with N.9, as observed in the neighboring State of São Paulo.

SARS-CoV-2 variant N. 9 identified in Rio de Janeiro, Brazil

BACKGROUND The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) B.1.1.33-derived lineage named N.9 was described recently in Brazil and it's considered a potential variant of interest (VOI) due to the presence of E484K substitution at the receptor-binding domain (RBD) of the Spike (S) protein. OBJECTIVE To describe the first detection of variant N.9 in Rio de Janeiro State. METHODS SARS-CoV-2 N.9 was confirmed by quantitative reverse transcription polymerase chain reaction (qRT-PCR), whole-genome sequencing and phylogenetic analysis. FINDINGS Here, we report two SARS-CoV-2 N.9 lineage strains in Rio de Janeiro. One of them had only the E484K substitution of the six N.9 lineage-defining mutations. Other three strains pre-defined as N.9 have the same genomic profile. These four strains are grouped within the B.1.1.33 lineage and basal to the N.9 lineage in our phylogenetic analysis, and we call them "N.9-like/B.1.1.33 + E484K". MAIN CONCLUSIONS The phylogenetic analysis shows four independent introductions of N.9 in the state of Rio de Janeiro in October and December 2020, January and March 2021. SARS-CoV-2 N.9 dissemination in the Rio de Janeiro could have been limited by the emergence and dominance of other variants, mainly by the lineage P.2 VOI Zeta that emerged in the same period and co-circulated with N.9, as observed in the neighboring State of São Paulo.

Peripheral venipuncture practices performed by nursing professionals in a university hospital / Práticas de venopunção periférica dos profissionais de enfermagem em um hospital universitário / Prácticas de venopunción periférica de profesionales de enfermería en un hospital universitario

Objective:The study's main purpose has been to characterize the peripheral venipuncture practices performed by nursing professionals providing care to pediatric and geriatric patients in a Brazilian Southern university hospital. Methods: It is a descriptive and observational study with a quantitative approach, which was performed in a Brazilian Southern university hospital over the period from December 2015 to June 2016, counting with 14 nursing professionals who were performing peripheral venipuncture in children and elderly people at the study's data collection time. Results: A total of 20 observations were made for the elderly group and 5 for the pediatric group. During all observations there was some divergence with the theoretical orientation that could result in harm to the patient and/or professional. Conclusion: By carrying out this study, it was noticed a variation of the technique and divergences between the practiced actions, therefore, underlining the importance of defining standard procedures and techniques based on scientific reasoning to promote patient safety

Objetivo: Caracterizar as práticas de punção venosa periférica executadas pelos profissionais de enfermagem no cuidado aos pacientes pediátricos e geriátricos em um hospital universitário do sul do país. Método: Quantitativo, observacional descritivo. Realizado em um hospital universitário da região sul do país, de dezembro de 2015 a junho de 2016, com 14 profissionais da equipe de enfermagem durante a realização da prática de punção venosa periférica em crianças e idosos. Resultados: Realizou-se 20 observações para o grupo de idosos e 5 para o grupo pediátrico, e em todas as observações houveram alguma divergência com a orientação teórica que poderia resultar em um dano ao paciente e/ou profissional. Conclusão: Ao realizar esse estudo, percebeu-se uma variação da técnica e divergências entre as ações praticadas, destacando a importância da padronização e desenvolvimento de técnicas a partir de fundamentação científica para promoção da segurança do paciente

Objetivo: Caracterizar las prácticas de punción venosa periférica que realizan los profesionales de enfermería en la atención de pacientes pediátricos y geriátricos en un hospital universitario del sur del país. Método: cuantitativo, observacional, descriptivo. Realizado en un hospital universitario de la región sur del país, de diciembre de 2015 a junio de 2016, con 14 profesionales del equipo de enfermería durante la práctica de venopunción periférica en niños y ancianos. Resultados: se realizaron 20 observaciones para el grupo de ancianos y 5 para el grupo de pediatría, y en todas las observaciones hubo alguna divergencia con la orientación teórica que podría resultar en daño al paciente y / o profesional. Conclusión: Al realizar este estudio se observó una variación de la técnica y divergencias entre las acciones realizadas, destacando la importancia de la estandarización y el desarrollo de técnicas con fundamento científico para promover la seguridad del paciente

Myristyltrimethylammonium Bromide (MYTAB) as a Cationic Surface Agent to Inhibit Streptococcus mutans Grown over Dental Resins: An In Vitro Study.

This in vitro study evaluated the effect of myristyltrimethylammonium bromide (MYTAB) on the physical, chemical, and biological properties of an experimental dental resin. The resin was formulated with dental dimetacrylate monomers and a photoinitiator/co-initiator system. MYTAB was added at 0.5 (G0.5%), 1 (G1%), and 2 (G2%) wt %, and one group remained without MYTAB and was used as the control (GCtrl). The resins were analyzed for the polymerization kinetics, degree of conversion, ultimate tensile strength (UTS), antibacterial activity against Streptococcus mutans, and cytotoxicity against human keratinocytes. Changes in the polymerization kinetics profiling were observed, and the degree of conversion ranged from 57.36% (±2.50%) for G2% to 61.88% (±1.91%) for G0.5%, without a statistically significant difference among groups (p > 0.05). The UTS values ranged from 32.85 (±6.08) MPa for G0.5% to 35.12 (±5.74) MPa for GCtrl (p > 0.05). MYTAB groups showed antibacterial activity against biofilm formation from 0.5 wt % (p < 0.05) and against planktonic bacteria from 1 wt % (p < 0.05). The higher the MYTAB concentration, the higher the cytotoxic effect, without differences between GCtrl e G0.5% (p > 0.05). In conclusion, the addition of 0.5 wt % of MYTAB did not alter the physical and chemical properties of the dental resin and provided antibacterial activity without cytotoxic effect.

A quantitative cross-sectional analysis of the melanin index in the skin of preterm newborns and its association with gestational age at birth.

BACKGROUND: Estimation of gestational age (GA) is important to make timely decisions and provide appropriate neonatal care. Clinical maturity scales to estimate GA have used skin texture and color to assess maturity at birth facing situations of the uncertainty of pregnancy dating. The size and darkness of the areola around the nipple to grade skin characteristics are based on visual appearance. The melanin index (M-Index) is an optical skin parameter related to the melanin content in the tissue. This study is aimed to associate the M-Index of the skin with the GA. METHODS: A cross-sectional study evaluated 80 newborns at birth. A photometer device quantified the skin pigmentation on the areolae, forearms, and soles. Paired average differences of M-Index were compared among the three body sites. The skin M-Indexes were compared between subgroups of newborns until 34 weeks or with 34 and more. RESULTS: The skin over the areola had the highest values of M-Index compared with the forearm or sole areas (P < .001 for both). Infants with a GA between 34 and <37 weeks had higher M-Index values over the areola than the group with a GA with 24 to <34 weeks: 41.7 (8.9) and 38.3 (10.5) median (IQR), P = .005. CONCLUSIONS: The measurable M-Index values have the potential to improve physical evaluation in assessing GA at birth.

Microfiltración apical entre dos cementos de obturación: biocerámico y resinoso en premolares unirradiculares preparadas con protaper, y obturadas con condensación lateral / Apical microfiltration between two filling cements: bioceramic and resinous in unirradicular premolars prepared with protaper and sealed with lateral condensation

Resumen Objetivo: Comparar in vitro la microfiltración apical en premolares unirradiculares obturados con cemento a base de resina epóxica y cemento biocerámico. Método: 40 piezas dentales unirradiculares fueron seleccionadas y divididas en dos grupos para ser obturadas esperando el tiempo de fraguado de acuerdo con las instrucciones del fabricante. El grupo A se obturó con cemento biocerámico Endosequence y, el grupo B, con cemento a base de resina epóxica AH-Plus: posteriormente los dos grupos fueron sellados con barniz de uñas hasta 3mm de la parte apical, luego se colocaron las muestras en la incubadora a 37ºC. Ambos fueron sometidos a 750 ciclos de termociclado, para reproducir el ambiente parecido a la cavidad oral. Para análisis de microfiltración se utilizó el método de difusión del colorante, se sumergieron en azul de metileno al 2 % por 6 días, posteriormente los especímenes fueron sometidos en una bomba de vacío. Los dientes fueron cortados longitudinalmente para ser evaluadas mediante el estereomicroscopio. El análisis estadístico fue hecho mediante T-Student y Chi-Cuadrado. Resultados: El grupo A corresponde a Endosequence obtuvo una medida de 0,55mm y el grupo B corresponde a AH-Plus 1,20mm con un estimado de significancia de p=0,013. Conclusión: El análisis de ambos cementos de obturación demostró que Endosequence posee menor microfiltración apical que AH-Plus.

Abstract Aim: To compare in vitro by the stereomiscroscope the lesser apical microfiltration in uniradicular premolars, between the cement based on Epoxy Resin and Bioceramic cement. Method: The study was performed on 40 uniradicular dental pieces divided into two groups, the samples were prepared using the protaper system and the obturation was performed using the lateral condensation technique, the first group was sealed withEndosequence bioceramic cement and the second group with Cement based on AH-Plus epoxy resin, then sealed with nail varnish up to 3mm from the apical part, then the samples were placed in the incubator at 37ºC, waiting for the set time corresponding to each group according to the manufacturer's instructions . The two groups of the present study were submitted to 750 cycles of thermocycling, to provide an environment similar to the oral cavity, then longitudinal cuts were made to the samples. The microfiltration was evaluated using the dye diffusion method, immersed in 2% methylene blue for 6 days, then the specimens were subjected to a vacuum pump. The teeth were cut longitudinally to be evaluated by the stereomicroscope. The statistical analysis was through T-Student and Chi-Square. Results: Group A corresponds to Endosequence obtained a mean of 0,55mm and Group B corresponds to AH-Plus 1,20mm with an estimated significance of p = 0,013. Conclusion: Both obturation cements have significant differences, therefore Endosequence has less apical microfiltration than AHPlus.

Níveis séricos de vitamina D em pacientes portadores de HIV e sua associação com fatores clínicos e nutricionais / Vitamin D serum levels in HIV patients and its association with clinical and nutritional factors

INTRODUÇÃO: A vitamina D está em evidência devido à sua relação com a promoção de melhor qualidade de vida, inclusive em pacientes com HIV, atribuído, principalmente, à sua atuação no sistema imunológico. OBJETIVOS: Verificar os níveis séricos de vitamina D e sua associação com os fatores clínicos e nutricionais em pacientes portadores de HIV. METODOLOGÍA: Estudo descritivo de corte transversal, realizado entre maio e dezembro de 2018, em um ambulatório do Recife, com pacientes com idade igual ou superior a 18 anos com diagnóstico de HIV que possuíam níveis de vitamina D dosados no último ano. Foram coletadas informações como idade, sexo, tempo de diagnóstico da infecção, medicações antiretrovirais, comorbidades e forma de transmissão do vírus. Para avaliação nutricional, foram mensurados peso, altura, índice de massa corporal, prega cutânea triciptal, circunferência do braço e circunferência muscular do braço. Foram registrados os seguintes exames bioquímicos: células de diferenciação 4, carga viral e perfil lipídico. Para a análise estatística utilizou-se o Programa SPSS versão 13.0. O projeto foi aprovado no Comitê de Ética em pesquisa para seres humanos, obtendo CAAE N° 76950417.9.0000.52.01. RESULTADOS: A amostra foi composta por 64 pacientes. Foi encontrado insuficiência de vitamina D em 35,9% dos participantes. De acordo com o índice de massa corporal, metade da amostra apresentou excesso de peso. Enquanto que, pelos outros parâmetros antropométricos, houveram classificações diferentes. A maioria dos participantes possuía bons níveis de células de diferenciação 4, carga viral e perfil lipídico dentro dos valores de referência. Não houve associação significativa entre hipovitaminose D e as variáveis independentes analisadas. CONCLUSÃO: Concluiu-se que a deficiência de vitamina D nessa população pode ser comum, assim como o excesso de peso, mostrando que nem sempre a desnutrição prevalece como diagnóstico nutricional nesses pacientes, principalmente quando estes fazem uso regular dos antirretrovirais e são acompanhados ambulatorialmente

BACKGROUND: Vitamin D is evidenced due to its relation with the promotion of a better quality of life, including in patients with human immunodeficiency virus, attributed mainly to its performance in the immune system. OBJECTIVES: To verify serum levels of vitamin D and its association with clinical and nutritional factors in patients with human immunodeficiency virus. METHODS: A cross-sectional descriptive study, conducted between May and December 2018, in an infectious disease outpatient clinic in Recife, Brazil with patients aged 18 years and over diagnosed with HIV who had vitamin D levels measured in the last year. Data such as age, gender, time of diagnosis of the infection, antiretroviral medications, comorbidities, and form of virus transmission were collected. For nutritional evaluation, weight, height, body mass index, triceps skinfold, arm circumference and arm circumference were measured. The following biochemical tests were also registered: differentiation cells 4, viral load and lipid profile. For the statistical analysis, the SPSS Program version 13.0 was used. The project was approved by the Research Ethics Committee for humans, obtaining CAAE No.76950417.9.0000.52.01. RESULTS: The sample consisted of 64 patients. Vitamin D insufficiency was found in 35.9% of the participants. According to the body mass index, half of the sample was overweight. While, by the other anthropometric parameters, there were different classifications. Most of the participants had good levels of differentiation 4 cells, viral load and lipid profile within the reference values. There was no significant association between hypovitaminosis D and the independent variables analyzed. CONCLUSION: It was concluded that vitamin D deficiency in this population may be common, as well as being overweight, showing that malnutrition does not always prevail as a nutritional diagnosis in these patients, especially when they take regular antiretrovirals and are followed up outpatient

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Can Children Have Ordinary Expectable Caregiving Environments in Unconventional Contexts? Quality of Care Organization in Three Mexican Same-Sex Planned Families.

The aim of this research was to explore the elements that configure the quality of care among three Mexican same-sex planned families: two female-parented families (through donor insemination) and a male-parented one (through adoption). The first family consisted of two mothers and a 3-year-old daughter; the second one had two mothers and a 1.5-year-old set of boy twins and the third family consisted of two fathers and a 2-year-old girl. It was assumed that Ainsworth's notions of quality of care organization are useful in order to understand caregiver-child attachment relationships, regardless of the parents' sexual orientation. A collective case study was selected due to the fact that these families shared their "unconventionality" (i.e., parents were not heterosexual) and the fact that they were planned, but each one constituted a particular case with a unique configuration. Four trained independent observers used the q-sort methodology (Maternal Behavior Q-Sort and Attachment Q-Sort) to describe parents' and children's behavior, respectively. The findings showed that parents were highly sensitive and all children used them as a secure base. To provide an in-depth examination of which elements configure the quality of care, a semi-structured interview with each parent was carried out. Through a thematic analysis, an over-arching theme named Affections and Emotions was identified, together with six subthemes: (1) Creating an affective environment; (2) Being available; (3) Acknowledging and expressing emotions; (4) Perceiving, interpreting and responding adequately to the child's real self; (5) Taking the child's perspective into account; and (6) Agreeing on roles and dividing the tasks. In order to showcase the particular configuration of gay parenting, the male-headed family narrative is reported in detail, because gay parents have been perceived as violating traditional gender roles as well as the hegemonic model of masculinity. The findings were consistent with the notion of quality of care as proposed by Ainsworth and her collaborators. The implications of the methodological device and research regarding same-sex planned families are discussed so as to understand the organization of the caregiving environment.

Comparación in vitro del sellado apical entre dos sistemas de obturación termoplastificada: Guttacore y E&Q Master / In vitro comparison of the apical seal between two thermoplastic filling systems: Guttacore and E&Q Master

Objetivo: Comparar el sellado apical in vitro de los sistemas de obturación termoplastificada, GuttaCore® y E&Q Master®, desde la perspectiva de microfiltración. Métodos: Cuarenta piezas unirradiculares se instrumentaron con sistema rotatorio e irrigaron con hipoclorito de sodio al 2,5% y EDTA al 17%. Las muestras se dividieron en dos grupos (n=20); se obturaron y colocaron en estufa incubadora a 37°C y 100% de humedad durante 3 días. Cada muestra se cubrió con barniz de colores excepto 3 mm apicales para ser sumergidas en azul de metileno al 2% por 3 días, y centrifugados por 20 min a 3000 rpm. Las muestras se lavaron y fracturaron axialmente para su análisis, mediante fotografías, en el estereomicroscopio. Para el análisis de datos se utilizó la prueba t de Student con un nivel de significancia del 5%. Resultados: Los valores medios de microfiltración fueron 0,660 mm y 0,825 mm para GuttaCore® y E&Q Master®, respectivamente, no encontrándose diferencias estadísticamente significativas entre ellas (p=0,525). Conclusión: GuttaCore® y E&Q Master® son sistemas de obturación que ofrecen un buen sellado apical no existiendo diferencia entre ellos. Palabras clave: EDTA; Gutapercha; Hipoclorito de Sodio; Microfiltración.

Objective: Compare in vitro the apical seal of thermoplastic filling systems, GuttaCore®and E&Q Master®, from the perspective of microfiltration. Methods: Forty single-rooted teeth were instrumented with rotary system and irrigated with 2.5% sodium hypochlorite and 17% EDTA. The samples were divided into two groups (20 for each system); sealed and placed in the incubator at 37°C and 100% humidity for 3 days. Each sample was covered with colored varnish except the last 3 apical millimiters to be immersed in 2% methylene blue for 3 days and centrifuged for 20 min at 3000 rpm. The samples were washed and fractured axially for their analysis in the stereomicroscope by means of photographs. For the data analysis we used the t Studenttest with a level of significance of 5%.Results: The mean microfiltration values were 0.660 mm and 0.825 mm for GuttaCore and E&Q Master respectively, no statistically significant differences were found between them (p=0.525). Conclusion: GuttaCore and E&Q Master are seal systems that offer good apical sealing, there is no difference between them. Keywords: EDTA; Gutta-Percha; Microfiltration; Sodium Hypochlorite.

The association between social skills deficits and family history of mood disorder in bipolar I disorder

Objective: To compare social skills and related executive functions among bipolar disorder (BD) patients with a family history of mood disorders (FHMD), BD patients with no FHMD and healthy control (HCs). Methods: We evaluated 20 euthymic patients with FHMD, 17 euthymic patients without FHMD, and 31 HCs using the Social Skills Inventory (SSI) and a neuropsychological battery evaluating executive function, inhibitory control, verbal fluency and estimated intelligence. Results: Both BD groups had lower SSI scores than controls. Scores for one subfactor of the social skills questionnaire, conversational skills and social performance, were significantly lower among patients with FHMD than among patients without FHMD (p = 0.019). Both groups of BD patients exhibited significant deficits in initiation/inhibition, but only BD patients with FHMD had deficits in verbal fluency, both compared to HC. There were no associations between social skills questionnaire scores and measures of cognitive function. Conclusion: Euthymic BD patients have lower social skills and executive function performance than HC. The presence of FHMD among BD patients is specifically associated with deficits in conversational and social performance skills, in addition to deficits in verbal fluency. Both characteristics might be associated with a common genetically determined pathophysiological substrate.